Use of Domain-Deletion Mutants to Locate Lutheran Blood Group Antigens to Each of the Five Immunoglobulin Superfamily Domains of the Lutheran Glycoprotein: Elucidation of the Molecular Basis of the Lu/Lu and the Au/Au Polymorphisms

Lutheran glycoprotein (Lu gp) has five predicted immuno4, and Lu13 and Au to domain 5. The organization of the LU gene was determined. The region encoding the open globulin superfamily (IgSF) domains. K562 cells were transfected with Lu cDNA and tested by flow cytometry with reading frame is arranged in 15 exons extending over ̧11 kb on chromosome 19q13.2. The Lu/Lu and Au/Au blood monoclonal antibodies and Lu blood group antisera. The results confirmed the identity of Lu cDNA. Deletion mutants group polymorphisms were studied using genomic DNA from typed blood donors. The Lu mutation is a base change lacking the regions encoding one or more IgSF domains were made by inverse polymerase chain reaction (PCR), exin exon 3 (G to A) encoding an Arg (Lu) to His (Lu) change on the CFG face of domain 1. The Au/Au polymorpressed in K562 cells, and tested with the same antibodies. The Lu and Lu5 antigens and the epitope recognized by phism is an A to G substitution in exon 12 encoding a Thr (Au) to Ala (Au) change on the G strand of domonoclonal antibody BRIC 224 were mapped to the first, Nterminal, IgSF domain. Lu4 and Lu8 were mapped to domain main 5. q 1997 by The American Society of Hematology. 2; Lu20 to domain 3; Lu7 and BRIC 221 epitope to domain